Friday, November 29, 2019
Vanity Fair, Bleak House And Felix Holt Essay Example For Students
Vanity Fair, Bleak House And Felix Holt Essay Queen Victoria and her consort, Prince Albert, established the idea of a sound family life as a prime value of the mid-century years. In Vanity Fair, Bleak House, and Felix Holt we see a very different idea in family rapport. In Vanity Fair, the Sedley family gives us a good example of a family that did not see sound family life as a prime value. Though both Amelia and Jos live good lives, Amelia going to a respectable girlsââ¬â¢ academy, Miss Pinkertonââ¬â¢s, and Jos living abroad in India, neither of them is exceptional, though, an outsider might have seen them so. Amelia, whom we see being well liked by many and a seemingly lovely girl, is not as perfect as she seems. A major fault that Amelia has is her ideas about love. Though we see this more as the novel progresses, we see her relationship with George and see it more as an infatuation then a genuine love. She is in love with the appearance of George and the idea of George more than the person that he actually is. In chapter 3 we see Jos cruelly tricking Becky into eating spicy food which she has never tasted before. As for Mr. Sedley, he is not a loving father. He is very mean to Jos, calling him names and making fun of him at any given oppor tunity. Mr. Sedley is thoughtless, nasty and cruel. To an outsider like Becky, oneââ¬â¢s initial reaction to the Sedleys is that they are an ideal family, but once one is submerged into their world he/she begins to see the truth behind their fa?ade. Becky also idealizes them initially because she is an orphan. She comes from a family less fortunate than the Sedleys and therefore sees them as a perfect. Now that she is alone she must work to take care of herself. She is very unhappy about this because she likes being with the Sedleys and also because she has set her sights on Jos as a potential mate. In Bleak House the first encounter we have with a family or non-family is with Sir Leicester and Lady Dedlock. Not only are they are thirty years different in age but they have no children. We can infer that this bothers Lady Dedlock because of her reaction to the neighboring family who seem loving and caring. We become acquainted with Esther was raised by her godmother who treated he r horribly. She constantly reminded Esther that she was her motherââ¬â¢s ruin because of the circumstances under which she was born. Esther grew up without love and feeling worthless. After her godmotherââ¬â¢s death she becomes a ward of the crown and is taken care of by Mr. Jarndyce. (There are also other ward of the crown, Rich and Ada, whom Mr. Jarndyce takes care of.)The Jellyby family is quite dysfunctional. Mrs. Jellyby is only interested in her charity work. She ignores her children and her home leaving them in squalor. The house and the children are filthy. The only child who she pays attention to is Caddy, whom she uses as her secretary. Mrs. Pardiggle also does charity work, missionary work to be exact. Her five sons dislike her and are ferocious with discontent because she forces them to do that work as well and to contribute money to it. We will write a custom essay on Vanity Fair, Bleak House And Felix Holt specifically for you for only $16.38 $13.9/page Order now One more example of a non-family situation is the Neckett family. After Mr. Neckett dies, Charlotte, better known as Charlie, must take care of her siblings. Though they adore her this is far from what we would see as the ideal family situation. .u5aca070c8be6bf4884d56d8507891961 , .u5aca070c8be6bf4884d56d8507891961 .postImageUrl , .u5aca070c8be6bf4884d56d8507891961 .centered-text-area { min-height: 80px; position: relative; } .u5aca070c8be6bf4884d56d8507891961 , .u5aca070c8be6bf4884d56d8507891961:hover , .u5aca070c8be6bf4884d56d8507891961:visited , .u5aca070c8be6bf4884d56d8507891961:active { border:0!important; } .u5aca070c8be6bf4884d56d8507891961 .clearfix:after { content: ""; display: table; clear: both; } .u5aca070c8be6bf4884d56d8507891961 { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .u5aca070c8be6bf4884d56d8507891961:active , .u5aca070c8be6bf4884d56d8507891961:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .u5aca070c8be6bf4884d56d8507891961 .centered-text-area { width: 100%; position: relative ; } .u5aca070c8be6bf4884d56d8507891961 .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .u5aca070c8be6bf4884d56d8507891961 .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .u5aca070c8be6bf4884d56d8507891961 .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .u5aca070c8be6bf4884d56d8507891961:hover .ctaButton { background-color: #34495E!important; } .u5aca070c8be6bf4884d56d8507891961 .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .u5aca070c8be6bf4884d56d8507891961 .u5aca070c8be6bf4884d56d8507891961-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .u5aca070c8be6bf4884d56d8507891961:after { content: ""; display: block; clear: both; } READ: Role of Ambition in Macbeth EssayIn Felix Holt the first family we encounter is the Transome family. They are quite dysfunction from the very beginning. We are told that Mr. and Mrs. Transome have not seen their son in many years nor have they ever met their grandson, this alone makes us begin to wonder what kind of familial ties they have. After Harold arrives he is nothing but mean and disrespectful to his mother and father. He arrives without his son, he bosses his mother around rearranging everyoneââ¬â¢s living quarters in the house to suit his own needs, and he makes fun of his father who is not well. Proving further the lack of family values in this household, we come to find out that Mr. Transome is not even Haroldââ¬â¢s father but the neighbor Mr. Jermyn in fact is. These examples taken from Vanity Fair, Bleak House, and Felix Holt show us that these novels, though written in the time of Queen Victoria, did not emanate the family values that she and Prince Albert tried to institute. The families portrayed in these novels are quite opposite. These families were far from having a sound family life; one might question calling them families at all. English Essays
Monday, November 25, 2019
Sociology Essay
Sociology Essay Free Online Research Papers Sociology is the study of social life, social change, and the social causes and consequences of human behavior. Sociologists investigate the structure of groups, organizations, and societies, and how people interact within these contexts. Since human behavior is shaped by social factors, the subject matter of sociology ranges from the intimate family to the hostile mob; from organized crime to religious cults; from the divisions of race, gender and social class to the shared beliefs of a common culture; and from the sociology of work to the sociology of sports. In fact, few fields have such broad scope and relevance for research, theory, and application of knowledge. (Hillsman, 2006) According to Wikipedia, Methods of sociological inquiry vary. The basic goal of sociological research is to understand the social world in its many forms. Quantitative methods and qualitative methods are two main types of sociological research. Sociologists often use quantitative methods, such as social statistics or network analysis to investigate the structure of a social process or describe patterns in social relationships. Sociologists also often use qualitative methods such as focused interviews, group discussions and ethnographic methods to investigate social processes. Sociologists also use applied research methods such as evaluation research and assessment. (Social Research, 2009) Wikipedia also discusses how sociologists use the internet with sociology. The Internet can be used as a tool for research (for example, conducting online questionnaires), a discussion platform, and as a research topic. Sociology of the Internet in the broad sense includes analysis of online communities (i.e. newsgroups, social networking sites) and virtual worlds. Organizational change is catalyzed through new media like the Internet, thereby influencing social change at-large. This creates the framework for a transformation from an industrial to an informational society. Online communities can be studied statistically through network analysis and at the same time interpreted qualitatively through virtual ethnography. Social change can be studied through statistical demographics, or through the interpretation of changing messages and symbols in online media studies. (Sociology on the Internet, 2009) Sociology is broken down into two categories. The first is Macro-Sociology (aka Structuralism). The individual is born into an ongoing social system, which exists independently of and determines his or her behavior. The individual acts accordingly to the script laid down by society. The values, institutions, and culture of society shape actions and roles. These are acquired in the process of socialization. This is a very deterministic approach whereby the point of departure is whole societies and the way they determine human behavior. (Viner, 2005) The second group is Micro-Sociology (aka the Social Action Approach). A human being is capable of conscious thought and self-awareness. Human action is not simply a reaction to external stimuli, but the result of the meanings, theories, motives, and interpretations brought into a social situation by the individual. Social reality is a constantly emergent property, not something fixed and inevitable. This is a voluntary approach stressing the individualââ¬â¢s voluntary actions. For example, ââ¬Ëfreewillââ¬â¢ is stressed. (Viner, 2005) As for my thoughts on sociology, I believe sociology has its place. I donââ¬â¢t think I could ever call it a proven science. There is a great deal of theory and statistics involved in sociology. I took a statistics class at Devry University a few months ago. There was definitely a lot of data involved in statistics. However, the data was based on samples of populations and populations that had data that was always changing due to individuals/objects that were being added and subtracted from data being calculated. You could never get a definite answer in statistics due to the data that constantly changed. I see the same thing with sociology. There isnââ¬â¢t one theory that we as humans can call the fact that explains everything. So, therefore the theory is used to explain our concept of sociology. Iââ¬â¢m always uneasy when the only explanations I have to explain something are a lot of theories. Below are some of the major general sociological theories (and their variants) include: Conflict theory: focuses on the ability of some groups to dominate others, or resistance to such domination. Ethnomethodology: examines how people make sense out of social life in the process of living it as if each was a researcher engaged in inquiry. Feminist theory: focuses on how male dominance of society has shaped social life. Functionalism: A major theoretical perspective which focuses on how elements of society need to work together to have a fully functioning whole. Interpretative sociology: This theoretical perspective, based in the work of Max Weber, proposes that social, economic and historical research can never be fully empirical or descriptive as one must always approach it with a conceptual apparatus. Social constructionism: is a sociological theory of knowledge that considers how social phenomena develop in particular social contexts. Social phenomenology: The social phenomenology of Alfred Schà ¼tz influenced the development of social constructionism and ethnomethodology. Social positivism: Social Positivists believe that social processes should be studied in terms of cause and effect using the scientific method. Structural functionalism: also known as a social systems paradigm addresses what functions various elements of the social system perform in regard to the entire system. Symbolic interactionism: examines how shared meanings and social patterns are developed in the course of social interactions. o Dramaturgical perspective a specialized symbolic interactionism paradigm developed by Erving Goffman, seeing life as a performance Rational choice theory: models social behavior as the interaction of utility maximizing individuals. (Social theory, 2009) As I was saying, there are way too many theories that sociologists use to try to explain the social science of sociology. Humans have lived on this planet for a little over 7000 years and all we have are theories to explain sociology. There arenââ¬â¢t enough absolutes! Iââ¬â¢m not saying that progress isnââ¬â¢t being made; I just think we should have more facts than theories. Hillsman, Sally (February 18, 2006). What is sociology?. Retrieved January 14, 2009, from Asanet.org Web site: asanet.org/cs/root/topnav/sociologists/what_is_sociology Sociology of the Internet. (2009, January 6). In Wikipedia, The Free Encyclopedia. Retrieved 01:46, January 14, 2009, from http://en.wikipedia.org/w/index.php?title=Sociology_of_the_Internetoldid=262222577 Social research. (2009, January 3). In Wikipedia, The Free Encyclopedia. Retrieved 01:47, January 14, 2009, from http://en.wikipedia.org/w/index.php?title=Social_researcholdid=261612037 Viner, David (2005). Brief introduction to the sociological perspectives on society. Retrieved January 14, 2009, from hewett.norfolk.sch.uk Web site: hewett.norfolk.sch.uk/CURRIC/soc/TM/intro.htm Viner, David (2005). Map of sociological theory. Retrieved January 14, 2009, from hewett.norfolk.sch.uk Web site: hewett.norfolk.sch.uk/CURRIC/soc/Theory.htm Social theory. (2009, January 11). In Wikipedia, The Free Encyclopedia. Retrieved 02:30, January 14, 2009, from http://en.wikipedia.org/w/index.php?title=Social_theoryoldid=263455519 Research Papers on Sociology EssaySociology is a ScienceGlobal Distributive Justice is UtopianIslamic Fundamentalism Replaces CommunismHas the British Welfare System beenThree Concepts of PsychodynamicDeontological Teleological TheoriesCo-Educational vs. Single Sex SchoolsPoverty, Violence and Conflict How are they Related?Joel Kovelââ¬â¢s The Enemy of NatureThe Colour Purple
Thursday, November 21, 2019
Video summary Movie Review Example | Topics and Well Written Essays - 250 words - 1
Video summary - Movie Review Example tions since cars will reduce hence creating more parking spaces and reduce the commotion: only those people who genuinely need to drive in New York will come with their cars. Firstly the implementation will lead to reduced air pollution, make pedestrian life better, save a lot of energy and probably slow down the global warming. Above all, it will improve how public realm of New York; how to use sign boards and create a favorable environment for everybody residing the city. Professor Shoup does not see the sense as to why New York is an expensive city and people pays high costs for residence while they park for free which should be the other way round. He compares New York with the city of Pasadena. Were after putting parking revenue, the city has become beautiful with money collected being used to clean the rods and pavements, pays for police services, lights the streets at night and generally unlacing the public wealth of the city. I think this is a quite brilliant idea and should be applied in all cities that are experiencing parking and traffic jams
Wednesday, November 20, 2019
The Priority for Glenmeadie's Innovation Efforts Essay
The Priority for Glenmeadie's Innovation Efforts - Essay Example Marketing experts should be aware that the product is the thing that theyââ¬â¢re selling, and innovation in this field is also necessary to build a customer base. The purpose of this essay is to explore how Glenmeadie can balance their innovation efforts between the product and new types of marketing in an effort to impress new customers and build a loyal customer fan base, by using the Ansoff and 8Ps frameworks to provide an illustration on how best to tackle the issues at hand. Glenmeadie The information provided by Nunes & Driggs (2006) paints an outline of Glenmeadie. As a Scotch whiskey distillery, Glenmeadie has won 7 gold medals in one season on a national and international scale, suggesting an upmarket taste and therefore suggesting a target market of whiskey connoisseurs. The marketing campaign led by Bob consists of international efforts to put on Tastemakers events in 25 cities, starting in New York. The aim is to spend $15,000 on each event, offering tastes of various whiskeys and bringing in efforts from an apprentice distiller and buyers from local distributors. Glenmeadie is also trying to give a more personal face to the brand, having interactive websites and creating loyalty card programs. ... Rayport, Stephen Dull and Joe Scafido. Scafiado, a member of the executive council at Dunkinââ¬â¢ brands, raises concerns about the fact that the front- and back-house innovations programs seem to be presented as separate enterprise. This is mainly because they seem to have separate functions in marketing, with product development being considered part of back-room company enterprises and marketing focusing on customer involvement and sales. However, this is a limited perspective on the matter. At Dunkinââ¬â¢ brands, Scafiado brought together the culinary team and the operating specialists into one department, meaning that any culinary development is being innovated simultaneously with efforts on how to present this to the company. Herman, who is president of Lebanon, agrees that Glenmeadie should be focusing efforts on product excellence as well as drawing customers in with these innovative marketing campaigns. After all, it is the product that is being sold, not the marketing scheme. Rayport, founder and chairman of Marketspace, sees things differently. Although Glenmeadie has reported some stress on research and development budgets, Rayport suggests that this can only be a positive thing, even for Ellis, the distiller. Rayport even goes as far to suggest that Ellisââ¬â¢ argument against expansion in marketing efforts is a paradox, as Ellis cannot have the freedom to create and innovate in the distillery without an expanded market and expanded profits. Dull, vice president of strategy at Greensboro, sees things a little differently. Dull suggests that Glenmeadie is a luxury product, as a whiskey, and therefore the aims of the company to branch into mass-marketing are a mistake. Dull suggests that there hasnââ¬â¢t been much of an
Monday, November 18, 2019
Democracy Promotion for Realism, Liberalism, Constructivism Essay
Democracy Promotion for Realism, Liberalism, Constructivism - Essay Example Realism believes that democracy promotion is suspicious because states cannot be certain of the true intentions of democracy-promoting states and because the existence of a democratic international system is impossible (ââ¬Å"Realism Introductionâ⬠8, 15). Realism does not think that democracy can be trusted because it is someone elseââ¬â¢s democracy. The U.S. is not only projecting values because its democracy promotion is projecting U.S. power too. At the same time, promoting democracy internationally is hardly attainable because there is no government authority that can enforce an international form of democracy (ââ¬Å"Realism Introductionâ⬠8). In particular, if the U.S. promotes democracy in the Middle East, and the projection of values can undermine the power structures in autocratic states, the latter would want to reduce American influence on cultural values and political systems. Others might create alliances to engage in war with more democratic states. The r esult could be violent conflicts where weak autocratic states attack weaker autocratic or more democratic states, in an attempt to block Americaââ¬â¢s democracy promotion in the region. Liberalism promotes democracy that could lead to a strong, economically-interdependent international society. Economic liberalism believes that trade and economic interdependence can build peace (ââ¬Å"Liberalism Introductionâ⬠6). U.S. democratic values that positively impact the motivation for regional and international economic agreements for involved states are needed to establishing interdependency.
Saturday, November 16, 2019
Cell Culture and Protein Detection
Cell Culture and Protein Detection Overview: This practical is split into three main parts, each part allows us to develop certain techniques. The first part of the practical is focused on the techniques needed to successfully carry out cell cultures. The second part is using an analytical technique known as ELISA this is used to measure the amount of proteins secreted by the cells that were cultured in part one. Lastly, part three focuses on western blotting, this is a technique used to measure proteins too but it differs from ELISA in that it measures proteins that are present inside the cell. The fundamental objective of this practical is to look at how Bacterial lipopolysaccharide (LPS) alters the growth and the expression of the smooth muscle cell à ±-actin (SMA) in vascular smooth muscle cells (vSMC) while also detecting and quantifying cell signalling molecules (JNKââ¬â¢s) and cytokine secretion (TNF-à ±) in vascular cells. Section 1 ââ¬â Subculture Objective: The aim of this part of the practical is to subculture the bovine aortic vascular smooth muscle cells and to count the cells and check for viability by counting the cells in suspension on a haemocytometer using a microscope. The cells are also treated with a bacterial antigen (LPS) to look at its effect on viability, differentiation state and the activation of intracellular signalling and secretion of proteins. Immunocytochemistry is also carried out in this part of the practical. Method: Trypsin is used to remove the cells from the bottom of the flask. When the cells have dissociated, medium which contains a trypsin inhibitor is added. The medium stops excessive trypsin action from damaging the cells. The cell suspension is first diluted using trypthan blue to allow viable cells which remain white to be distinguished from non- viable cells which are blue. This method allows us to get a total cell count of cells/mL and the percentage of viable cells. Our total cell count was found to be 7.45105 cells/mL The non-viable cells were found to be 1.5104 cells/mL This means that the culture contains more 98% viable cells which indicates that it is a healthy culture. 3 different 6 well plates were then set up using different densities of seed cells. 3105cells/ml seeding density was used in one plate. This density was used as a lot of protein needs to be present for the assessment of JNK and à ±-actin protein cytokine production. 1105 cells/ml seeding density was used in another 6 well plate. These plates are used to assess the effect of LPS on growth and viability of the cells. This density is used to give a clear picture of the cells to make counting easier. 5103 cells/ml seeding density was used in the last plate. This plate is used for à ±-actin expression by immunocytochemistry. The low density will give us a clearer picture. The three different sets of plates are all treated with varying concentrations of LPS. Each plate has two wells which are used as controls, containing no LPS, two wells containing 1à µg of LPS and two wells containing 10à µg of LPS. Immunocytochemistry is carried out on the plate with a seeding density of 5103cells/ml. This technique is used to determine if a particular protein or antigen is present. An unlabelled primary antibody is used to bind to the antigen desired antigen. The presence of contractile protein à ±-actin can determine the differentiation state of vSMC. Immunocytochemistry is used as a qualitative method of determining the presence of a protein, it is not quantitative. Results: Calculations for different seeding densities: Initial concentration = 7.45105 cells/mL Formula = Plate 1, seeding density 1.5105 cells/ml Plate 2, seeding density 0.5105 cells/ml Plate 2, seeding density 2.5103 cells/ml Table 1: Cell growth for each group Immunochemistry results: Figure 1: Cell growth in the control (0à µg/ml LPS) Figure 1: Cell growth in the control (1à µg/ml LPS) Figure 3: Cell growth with 10à µg/ml of LPS Discussion: The first part of this section was to carry out a cell count and determine the viability of the suspension. Our sample had 7.45105 cells/ml and it contained more than 98% viable cells. This meant it was a healthy cell suspension and it was suitable to run tests on for the practical. A seeding density of 1105 cells/ml seeding density was used to assess the effect of LPS on growth and viability of the cells. This density was used to give a clear picture of the cells to make counting easier. The results of our experiment correlated with the majority of the class. However there were some unexpected results in some of the groups but this could be down to plates being labelled incorrectly or mistakes while counting when using the haemocytometer. Immunocytochemistry is used to allow us to analyse the results visually. We found that as the amount of LPS increased the number of cells decreased, this is illustrated in figures 1, 2 and 3. LPS is an endotoxin and it inhibits the growth of à ±-a ctin. LPS also damages the structure of à ±-actin, high levels of LPS stop the correct formation of the filaments and so affects the function of the cell which is to facilitate cell contraction and migration. This result was expected as LPS is a major mediator to septic shock and is known to directly affect vascular smooth muscle cells. Question: What could you do to improve this experiment? To improve this experiment I would use a wider range of concentrations for LPS. This would give a better understanding of its affects. Repeating the experiment several times and getting an average of your results would also help. Section 2 ââ¬â ELISA Objective: The aim of this section of the practical is to use Enzyme-Linked Immunosorbent Assay (ELISA) to detect production of the cytokine TNFà ± from the cells activated with LPS. Detection is based on a colour change. Firstly a standard curve must be generated so that absorbance values can be converted into concentrations of TNF-à ±. Once the curve is completed we can determine the unknown concentrations of TNF-à ± in our samples. Method: Firstly the antibody is immobilised onto the surface of the plate. The plate is then washed to remove any excess antibody, antigen is then added and it is allowed to bind to the antibody. A secondary antibody is then added, this antibody is labelled with an enzyme. The enzymes substrate is then added, this causes a colour change. The amount of coloured product formed is determined spectrophotometrically. The amount of coloured product is proportional to the amount of enzyme present and also to the concentration of the antigen. Results: Table. 2 Absorbance values of samples at 450nm. Figure 4. Plot of standard curve of absorbance versus concentration. Discussion: The aim of this experiment was to quantify the amount of TNF-à ± present in our samples. To do this a set of known standards were used and their absorbance values read. This data produced a straight line with an R2 value of 0.99 indicating that a straight line was an excellent fit for absorbance versus concentration, and so the equation of the line could be used to determine unknown concentrations of TNF-à ± based on their absorbance values. Our results showed that TNF-à ± was present in its highest concentration of 25.15pg/ml in the sample with the highest concentration of LPS and it was found in its lowest concentration of 9.9 pg/ml in the sample containing no LPS. This result was expected as cytokines such as TNF-à ± are produced in large quantities to respond to endotoxins such as LPS. Question: What could you do to gain more information from this experiment? To gain more information from this experiment you could test for other cytokines which are also activated by LPS, correlating these results would make your data more meaningful. Section 3 ââ¬â Western Blotting Objective: The aim of this section of the practical is to prepare cell lysates from the vascular smooth muscle cells which were activated by LPS previously. SDS PAGE and western blotting will then be used to detect the activation of the intracellular protein JNK. Western analysis quantifies the amount of protein present in the cell. To do this cell lysis must be carried out. In this practical we used a method which generates whole cell lysates. To do this lysis buffer is added to the cells followed by sonication. Method: Cell lysis is carried out first. The next step is SDS polyacrylamide gel electrophoresis. Western blotting involves transferring the protein bands from an acrylamide gel to a more stable and immobilising medium such as nitrocellulose paper so that analytical procedures such as detection with antibodies can be carried out. We carried out western blotting using iBlot dry blotting system. After blotting probing is carried out to determine the presence of phosphorylated JNK protein. Results: Figure 5. Ponceau S Staining Figure 6. à ±-actin Figure 7. pJNK Discussion: To see if our transfer was a success before probing, the blot was stained with Ponceau S stain. The proteins can be seen as red bands with this stain. The result of this stain can be seen in figure 5. red bands are present which indicates our transfer was a success and that there are proteins present. The western blot analysis showed that à ±-actin was present in all the samples as a strong band around 42kDa was observed which is expected for à ±-actin. The results for pJNK did not work out as it was washed incorrectly, because of this no bands were observed, however bands would be expected in the samples containing LPS. Why did you run the sample on the gel before blotting? The sample was run on gel first as proteins are separated by molecular weight. This allows us to distinguish à ±-actin from other proteins. It is then moved to the nitrocellulose paper so that analytical procedures such as detection with antibodies can be carried out Why measure the phosphorylated form of JNK? The phosphorylated form of JNK is a signal a cell sends out when it is stressed. Therefore pJNK should be present in the samples with LPS. If it is present then it confirms the fact that the cell is stressed as a result of the presence of the endotoxin.
Wednesday, November 13, 2019
Poor Parents Must Educate Their Children Essay example -- Solutions to
A child does not show up for school for the third day in a row and the teacher notices that a pattern of absences has appeared. Is it the childââ¬â¢s fault? The parentsââ¬â¢ fault? Can the school do something to stop this trend? There is a definite association between the parents of a child in poverty and the education that child does (or does not) receive, and there are many factors that play into this connection: intimidation the parents feel, expectations put on the child, parent employment, location and condition of the school, and health issues. Unfortunately, all of these issues mean that children in poverty are on an unequal plane when it comes to education, compared to children in higher classes of socio-economic status. Lord Acton wrote of the United States over 140 years ago, ââ¬Å"In a country where there is no distinction of class, a child is not born to the station of its parents, but with an indefinite claim to all the prizes that can be won by thought and labor . It is in conformity with the theory of equality . . . to give as near as possible to every youth an equal state in life. Americans are unwilling that any should be deprived in childhood of the means of competition.â⬠1 It is sad and ironic how this statement is not true in the United States today. Parents may not want to get involved in their childââ¬â¢s school life because schools have a negative connotation in their minds. Many adults in poverty grew up in poor families and have bad memories of school from their childhoods. They may have been bullied or did not make good grades. There may be an association for them between school and feeling stupid. Also, some parents of students in poverty have a lower level of education than the people working at schools. When talking to principals or teachers, a parent does not want to feel inferior in the conversation. The more diverse vocabulary used by the faculty and staff at schools can be intimidating. This is one reason why parents are reluctant to get involved in reading programs, the Parent Teacher Association, and other extra-curricular activities. In order to distance themselves from the feeling of inferiority, parents distance themselves from their childrenââ¬â¢s schools. In addition to being physically distant from the place their children receive their education, parents of children in poverty may not breach the topic verbally. Parental encouragement ... ...91. Philipsen, Maike Ingrid. ââ¬Å"The Problem of Poverty: Shifting Attention to the Non-Poor.â⬠In Late to Class: Social Class and Schooling in the New Economy, edited by George Noblit and Jane A. Van Galen, 269-285. Albany: State University of New York Press, 2007. Polakow, Valerie. ââ¬Å"In the Shadows of the Ownership Society: Homeless Children and Their Families.â⬠In Invisible Children in the Society and Its Schools, edited by Sue Books, 39-62. Mahwah, NJ: Lawrence Erlbaum Associates, 2007. Pollitt, Ernesto. ââ¬Å"The Relationship between Undernutrition and Behavioral Development in Children.â⬠The Journal of Nutrition Vol. 125, No. 8S (August 1995). http://www.unu.edu/ (accessed on April 6, 2015). Sato, Mistlina, et al. ââ¬Å"Poverty and Payne: Supporting Teachers to Work with Children of Poverty.â⬠Phi Delta Kappan Vol. 90, No. 5 (January 2009): 365-370. http://vnweb.hwwilsonweb.com (accessed April 6, 2015). Shipler, David K. The Working Poor: Invisible in America. New York: Random House, Inc., 2004. Weissbourg, Richard. ââ¬Å"The ââ¬ËQuietââ¬â¢ Troubles of Low-Income Children.â⬠The Education Digest Vol. 74, No. 5 (January 2009): 4-8. http://vnweb.hwwilsonweb.com (accessed April 6, 2015).
Monday, November 11, 2019
History of Psychology-John Locke and John Mill Essay
Psychology as a recognized science is a fairly new revelation, falling into favoritism only within the past century. Philosophy bears the roots of psychology, and philosophers have been pondering the inner workings of the mind and body for many centuries before psychology came in to favor. One of the key philosophers crucial in the development of psychology in to a formal discipline was Descartes. Their writings influenced more modern Western philosophers such as John Locke and John Mill. From this point, psychology grew into the science it is now recognized as. The Philosophers The 17th century is the cornerstone of philosophy evolving into psychology. Rene Descartes (1596-1650) is often considered to be the inspiration for and credited to be the father of our modern philosophy, and psychology. He is most famously quoted for ââ¬Å"I think, therefore I amâ⬠(Descartes, 2006). A humanââ¬â¢s ability to reason is the only to find truth. Descartes would also not accept anything to be truth unless there was no discernable reason for doubt. ââ¬Å"The only way to get to the certainty of truth is to arrive at oneself, relying on the clear use of oneââ¬â¢s own reasoning powersâ⬠(Goodwin, 2008, p.33). Disenchanted with the philosophy he was taught, Descartes found nothing in his learning that could not be left to question. He was said to only believe as truth what could not be doubted and Descartes pursued to find the knowledge he could find within himself (Goodwin, 2008). Descartes was a rationalist and dualist, making him firm believer in the mind and body being two completely separate entities. His beliefs on this are debatable in philosophy and psychology and have been so in the centuries following his life. Philosophers and psychologists to this day are still trying to find the connection between the mind and the body. Western Influences John Locke John Locke was a 17th century British philosopher who is credited with being the founder of Empiricism, the idea that our experiences are our only true source of knowledge. Lockeââ¬â¢s view on the formation of ideas was sensation and reflection is the only foundation on which we form ideas. He believed we acquired all of our knowledge form our experiences in the world and in life. Locke compared the human mind to a sheet of white paper. Over the course of life, all of our experiences are recorded upon this paper, our sensations. These sensations can later be recalled, in reflection, to add to new sensations and to acquire new compounded knowledge. John Mill John Mill is considered to be the most important contributor during the British Empirical movement. His contribution stated all knowledge is attained through experience and inborn ideas do not exist. Mill also believed a man should be able to do anything desired as long as the actions do not harm or hurt fellow man in the process. This also affected his views on the necessity of government. ââ¬Å"The only purpose for which power can be rightfully exercised over any member of a civilized community against his will is to prevent harm to others. His own good, either physical or moral, is not a sufficient warrant. â⬠(Mill, 2008). Mill found the government to only be necessary for the protection of the people in defense of a war, safety against violence, and to prevent cheating or fraud. Modern Psychology The evolution of philosophy into the science of psychology rapidly took shape in the 19th century. The world was also rapidly changing; the Industrial Revolution created new technologies to help in the advancement of many facets of science. Moving into the next century, advances in the knowledge in anatomy and of the brain further assisted the progress of psychology being a science. Robert Watson was crucial in the study of the history of psychology. Founding a new division of the American Psychological Association, he encourages a study of the history of psychology. As a result, nearly all psychology programs today require a course in psychologyââ¬â¢s history. (Goodwin, 2008) Conclusion. Psychology as the science we recognize today is barely more than a century in the making. When following the links to the past and the minds that have gotten us to where we are in psychology today, much it to be learned. From modern medicine and science all the way back to ancient philosophers, psychology is a longstanding and diverse study. References Descartes, Rene (2006). A discourse on method/ by Rene Descartes [Kindle DX version]. Retrieved from Amazon. com Goodwin, C. J. (2008). A history of modern psychology [3rd ed. ]. Mill, John S. (2008). On liberty/ by John Stuart Mill [Kindle DX version]. Retrieved from Amazon. com.
Saturday, November 9, 2019
A.G. Edwards Inc. Essays - Primary Dealers, Subprime Mortgage Crisis
A.G. Edwards Inc. Essays - Primary Dealers, Subprime Mortgage Crisis A.G. Edwards Inc. Business Profiles in American Enterprise A.G. Edwards Inc. The industry for securities is undoubtedly an exciting and fast paced industry. This means that brokerage firms such as A.G. Edwards and Sons must always be watching the stock prices on every stock in the market so that they can give their clients maximum profit. When A.G. Edwards and Sons clients do well then in turn so does the brokerage firm. A.G. Edwards Inc. is not the biggest corporations in America, but yet it is still a very large corporation and has great importance in the industry for which it participates. This paper will give an in depth explanation about how A.G. Edwards functions as a cor-poration. Along with competition from the government, banks and other brokerage firms there is also probably the biggest factor involved of interest rates. Interest rates are indi-rectly proportional to the activity in the stock market. This means that when the interest rates fall the market for securities becomes active. This is due to the fact that people want the highest yield on there money and when interest rates are low, investing money into a bank would yield less money then it would have before at a higher interest rate. So people tend to want to put there money into something that will give them a higher yield and stocks are just that. An example of this inversely proportional relationship is always being demon-strated and was demonstrated in the past few years. At the end of 1992 to the beginning of 1993 the volume in most businesses was at record levels obtaining a pre-tax net income for the whole industry of 9.1 billion dollars setting a new record for the second year in a row (Hoovers Company & Industry Database, 1993, p. 1 (Hoover, 1993,p.1)). This trend continued when in the beginning half of 1993 offerings (new business for the com-pany) exceeded those of 1992 (Hoover,1993, p. 2). Examples of this are as follows; more than 700 billion dollars of debt was issued in 1992 and then in the first half of 1993 an-other 440 billion dollars of debt was issued (Hoover, 1993, p. 2). More than half of this debt was due to asset-backed debt such as credit cards and other charges made to credit (Hoover, 1993, p. 2). These debts were included because the debts were more or less sold to banks and other money lending institutions who were more willing to take the risk for the high interest rate. This drop in interest rates did wonders for the brokerage firms involved and also corporations that had acquired debt over the years. The fall of interest rates was great for the brokerage firms because of the increase in business with the publics desire to invest. So the corporations used it to issue off more stock to the public to pay off their debts (Hoover, 1993, p. 2). As if interest rates didnt have enough effect on brokerage firms, there is also the heavy competition that was involved. This competition is not only from other top broker-age firms such as Merrill Lynch, Morgan Stanley, Primerica (Smith Barney Shearson), Salomon, and Goldman Sachs, but there is also competition from big banks and securities over seas. Banks have a number of ways to compete with security firms, but the most prevalent and direct is through mutual funds. Since the late 1980s banks have been ag-gressively competing with mutual fund sponsors by issuing there own mutual funds to the public (Hoover, 1993, p. 3). Mutual funds that are issued by banks are now the fastest growing part of the mutual fund industry, with 10.6 percent of total assets and 30 percent of new sales (Hoover, 1993, p. 3). There has just recently been a large amount of compe-tition from markets overseas and this competition continues to increase. There are a few theories as to what has caused so many U.S. investors to invest in foreign markets. It is supposed that it is either individual investors who want to further their portfolio or in-vesting in foreign markets to try and avoid interest rates and changes in currency (Hoover, 1993, p. 4). The investment in foreign markets has also been attributed to technology and the fact that up to date information can be obtained instantly from any place in the world (Hoover, 1993, p. 4). But whatever has caused it global securities have skyrocketed. Before the organization and structure of the company a brief history and explana-tion of the company will be discussed. A.G. Edwards & Sons was founded in
Wednesday, November 6, 2019
Reflexology Essay Example
Reflexology Essay Example Reflexology Essay Reflexology Essay Reflexology, or zone therapy is an alternative medicine involving the physical act of applying pressure to the feet, hands, or ears with specific thumb, finger, and hand techniques without the use of oil or lotion. It is based on what reflexologists claim to be a system of zones and reflex areas that they say reflect an image of the body on the feet and hands, with the premise that such work effects a physical change to the body. [1] A 2009 systematic review of randomised controlled trials concludes that The best evidence available to date does not demonstrate convincingly that reflexology is an effective treatment for any medical condition. [2] The Reflexology Association of Canada defines reflexology as: A natural healing art based on the principle that there are reflexes in the feet, hands and ears and their referral areas within zone related areas, which correspond to every part, gland and organ of the body. Through application of pressure on these reflexes without the use of too ls, cremes or lotions, the feet being the primary area of application, reflexology relieves tension, improves circulation and helps promote the natural function of the related areas of the body. [3] There is no consensus among reflexologists on how reflexology is supposed to work; a unifying theme is the idea that areas on the foot correspond to areas of the body, and that by manipulating these one can improve health through ones qi. [4] Reflexologists divide the body into ten equal vertical zones, five on the right and five on the left. [5] Concerns have been raised by medical professionals that treating potentially serious illnesses with reflexology, which has no proven efficacy, could delay the seeking of appropriate medical treatment
Monday, November 4, 2019
Cardiovascular Fitness (Designing an Experiment) Essay
Cardiovascular Fitness (Designing an Experiment) - Essay Example Cardiovascular Fitness (Designing an Experiment) Cardiovascular diseases have been associated to the increasing cases of obesity among children and adolescent as they approach adulthood (Olshanky and others 2005) cited in. However, little attention has been focused towards establishing the effect of excessive body fat and its negative impact on cardiac fitness (Rowland, 2007). Due to excessive fats there are high risks of coronary artery conditions such as renal-vascular diseases, hypertension and stroke coupled to other non-circulatory disorders. Body fatness and aerobic fitness are intertwined and have been reported as potential risk factors towards the health outcome of an individual (Goran and others 2000). Among the two factors, aerobic fitness has been reported as the primary factor determining future outcome of diseases (Goran and others 2000). However, it is important to understand the relationship between aerobic fitness and fatness towards establishing their role in causation of cardiovascular diseases. Cardiovascular risk has also been linked to weight and dissociated from the widely accepted norm that being fat and fit is acceptable in cardiac health. Excess weight in most cases may be associated with high levels of cholesterol and alteration in the cardiac function. 1.Ha: Excess weight affect cardiovascular fitness by increasing the pulse rate and recovery time. 2.Ho: Excess weight will not affect cardiovascular fitness The experiment involved two subjects who were selected from among the group. The inclusion criteria dwelt on involving subjects who had respiratory and/or circulatory conditions. Exercise which was part of the experiment may be significantly compromised in these individuals. Pulse rate for treatment I (with weight) and for treatment II (without weight) were taken before the start of the experimental exercise and entered in a table. The experimental exercise involved making the subject step up and down for three minutes at constant rate of 24-30 steps per minute on a low platform. Immediately the 3 minutes were over, the pulse rates were recorded again and entered into a table as raw data for further analysis. The pulse rates were taken from the radial artery. This was done by making a tally of the number of beats in 30 seconds and multiplying this value by two. Subsequent measurements were taken at intervals of 1 minute for another three times. 3. Result text The pulse rate before th e exercise was slow but it was elevated following the 3 minutes exercise in the two treatments. However, the mean pulse rate in treatment two was higher than in treatment one (see table 1). The trend in pulse rate indicates that individuals with excess weight will have high pulse rate and longer recovery time after exercise (see figure 1 and figure 2). Mean recovery time was higher in treatment II at 3.25 minutes as compared to treatment I with 2.50 minutes. Overall standard error from the mean (SEM) was observed to at the highest from pulse measurements taken after the exercise. This value was higher in treatment I than in treatment II. Overall all treatment I recorded elevated means in the measurements of pulse rate before and after the exercise than treatment II without weight. This trend was also replicated in the recovery time with
Saturday, November 2, 2019
Martha Stewart and her Insider Trading Essay Example | Topics and Well Written Essays - 1250 words
Martha Stewart and her Insider Trading - Essay Example The professionals at Martha Stewart Living Omnimedia generate a broad compilation of ââ¬Ëhow toââ¬â¢ articles, books, television programs, newspaper columns, radio sections and products relating to the eight core content areas all through the four business section. Publishing is Martha Stewart Living Omnimedia's largest business section that accounted for US $182.6 million of total revenues for 2002. It includes magazines such as Martha Stewart Living and Martha Stewart Weddings, nearly 40 Martha Stewart books, the ask Martha newspaper column and radio show, and a music library of compilation CDs. The Television section accounted for US $ 26.7 million of total revenues for 2002, where it now offers around 30 opportunities every week for viewers to watch Martha Stewart programming. Kmart plays a huge role in Martha Stewart Living Omnimedia's merchandising section. In 1987, Martha Stewart Living Omnimedia and Kmart created a partnership that has been extremely beneficial for both companies. Sales of Martha Stewart merchandise in Kmart stores have reportedly been in excess of US $5 billion since the beginning of the partnership. (Louria, 2) In 2001, Kmart sold US $1.5 billion worth of Martha Stewart Everyday house wares, and those licensing fees a ccounted for almost 25% of Martha Stewart Living Omnimedia's profits. But since Kmart filed for bankruptcy on January 22, 2001, Martha Stewart Living Omnimedia finds its fastest growing section - merchandising - in jeopardy. Kmart has increasingly lost ground to competitors such as Wal-Mart and Target due to ill-advised price-cutting strategies and a mismanaged supply chain. It has already closed the doors of 250 to 350 of 2,100 stores and Martha Stewart Living Omnimedia has taken quite a hit. ... It has already closed the doors of 250 to 350 of 2,100 stores and Martha Stewart Living Omnimedia has taken quite a hit. In many ways, the IPO for Martha Stewart Living Omnimedia, Inc. has thus far been the high watermark for both the organization and its principal stockholder, Martha Stewart. It was Martha's singular good fate-as well as bad fate-to go public with her stock offering at nearly the top of the greatest bull market in American history. This timing meant that while she immediately became a billionaire on paper, the stock would likely drift down with the rest of the market, so that, financially speaking at least, she had no where to go but down as well. Not only has she so far been unable to hold on to the initial phenomenal gains from the IPO itself, but also there have come to be other-and more troublesome-questions clouding the organization's future. One question had been hovering over the organization from the day it went public: What would happen to the shares if Martha herself-who held more of them than anyone else-were to meet an untimely end It was the same question that some investors had tossed out regularly during the IPO road show. (Louria, 3) Now that the stock was trading publicly, it was no longer a matter of mere theoretical concern: More than a billion dollars of stock market wealth hung on the ability of the organization's chairman to bolt from bed each morning and begin pursuing one of the most demanding schedules of any public figure on earth. And there was another problem that abruptly moved from the realm of the theoretical to the real: Would Martha's organization be able to survive looming problems besetting its most lucrative business partner, Kmart Corp. The entire matter became real, immediate, and suddenly
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